Tuesday, May 24, 2022

Pcr Blood Test For Lyme Disease

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If The C6 Test Is Positive What Is The Next Step

Lyme Disease Blood Tests part 1

A positive C6 test means antibodies to C6 were found. The next step is to do a QC6 test, which determines if the levels of antibody are high enough to justify treatment. If the value of the QC6 is higher than 30 IU/mL and signs of illness are present, then antibiotic treatment should be considered. If the QC6 is less than 30 IU/mL and there are no signs of illness, then treatment may not be necessary.

In addition to doing the QC6 test, your veterinarian may want to take samples of blood and urine to assess kidney function and to look for protein in the urine. A positive test for protein in the urine could signal serious underlying kidney disease.

Where Is Lyme Disease Found

In the United States, Lyme disease has been reported in every state, but over 95% of cases are from the Northeastern, Mid-Atlantic, and upper Midwestern states, with a small number of cases reported along the West Coast, especially Northern California. In Canada, Lyme-positive dogs are found mostly in southern Ontario and southern Manitoba, with a small number of cases in southern Quebec and the Maritime provinces.

Serological Testing Of Serum For The Diagnosis Of Lyme Disease

The most commonly used tests look for antibodies to the Borrelia species that cause Lyme disease in the UK and Europe, but they also detect infections from strains of Borrelia from the US.

The antibody response takes several weeks to reach a detectable level, so antibody tests in the first few weeks of infection may be negative. If the first sample was taken within 4 weeks from the start of symptoms and is negative and there is a clinical suspicion of Lyme disease, then retesting in 4 to 6 weeks may be useful.

It is very rare for patients to have negative antibody tests in longstanding infections. Borrelia antibodies persist indefinitely in some patients and this does not indicate continuing disease or a need for re-treatment.

Serological testing for Lyme disease in the UK and much of the world follows a two-step approach using commonly available antibody screening tests as a first stage, followed by immuno-blotting of samples that give positive or indeterminate results in the screening tests.

  • Sensitive screening tests are used at the first stage of testing but have the disadvantage of occasionally detecting other diseases and producing false positive results. RIPL uses the C6 Lyme ELISA for screening

  • Samples giving positive or indeterminate preliminary screening test results are then tested in a more specific system to confirm the presence of Borrelia-specific antibodies. RIPL uses the Borrelia ViraChip® IgG, IgM test for this purpose

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    Alternative Diagnostic Tests Not Available At Ripl

    Tests used in the NHS and PHE to identify Lyme disease are well characterised, standardised, and are highly reproducible between laboratories. They are the methods of choice recommended in the 2018 NICE Lyme disease guideline, following an extensive review of the evidence and literature. International external quality assurance schemes are in place to ensure consistency between different centres offering these tests.

    Several private laboratories in Europe and the USA offer an alternative type of test called an ELISpot to diagnose Lyme disease. This looks for different markers in blood samples compared to conventional validated Lyme disease serology tests. The laboratories using these tests in the diagnosis of Lyme disease do not publish their methods, and have not produced any peer reviewed publications on their clinical value.

    This makes it very difficult to verify their results, especially as there are no national or international EQA schemes for Lyme disease ELISpot tests and therefore no independent verification of performance between laboratories. Without independent evidence it is impossible to determine the validity of results produced using these alternative tests.

    RIPL cannot interpret the results of alternative diagnostic tests.

    Question 10 What Other Infections Can A Tick Transmit To Humans Besides Lyme Disease

    Lyme Disease PCR Blood Test

    I scapularis ticks can also carry other human pathogens that have a high degree of symptom overlap, including Borrelia miyamotoi, Anaplasma phagocytophilum, Ehrlichia chaffeensis, and Babesia microti.2,10-12 Several studies on co-infected ticks indicate as many as 20% of Ixodid ticks can be coinfected with B burgdorferi and one or more of these other tick-borne human pathogens.10-12

    Molecular tests can be useful to detect these organisms in early/acute stages of infection, when genetic material from the pathogen can be detected but antibodies remain below the limit of detection of serologic assays.2,10-12 Quest offers molecular and/or serological tests for B burgdorferi, B miyamotoi, A phagocytophilum, E chaffeensis, and B microti, individually and in 2 panels .

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    Is There A Blood Test For Lyme Disease

    If your doctor suspects that you have Lyme disease, they may order two blood tests. These will look for signs that your body is trying to fight it off. The results are most precise a few weeks after youâve been infected.

    These tests are:

    ELISA test. This test canât check for the bacteria that causes Lyme disease. It can only look for your immune systemâs response to it.

    Once Borrelia burgdorferi gets into your blood, your body begins to make special proteins called antibodies to fight it off. The ELISA test checks for those antibodies.

    Although itâs the most common way to check for Lyme disease, the ELISA test isnât perfect. It can sometimes give false âpositiveâ results. On the other hand, if you have it done too soon after youâve been infected, your body may not have developed enough antibodies for the test to detect them. This will give you a ânegativeâ result even though you do have Lyme disease.

    Western blot test. Whether your ELISA test comes back positive or negative, your doctor will need to do this blood test, too.

    A Western blot uses electricity to split certain proteins in your blood into patterns. This is then compared to the pattern of people known to have Lyme disease.

    At least five band matches means that you have Lyme disease. Still, not all labs have the same standards. Thereâs a chance that you could get a âpositiveâ result from one and a ânegativeâ result from another.

    Interpreting The Igm Western Blot Test: The 1

    If clinical symptoms and signs of Lyme disease have been present for more than 1 month, IgM reactivity alone should not be used to support the diagnosis, in view of the likelihood of a false-positive test result in this situation. This is called the 1-month rule in the diagnosis of Lyme disease.

    In early localized infection, Western blot is only half as sensitive as ELISA testing. Since the overall sensitivity of a 2-step algorithm is equal to that of its least sensitive component, 2-tiered testing is not useful in early disease.

    Although currently considered the most specific test for confirmation of Lyme disease, Western blot has limitations. It is technically and interpretively complex and is thus not universally available. The blots are scored by visual examination, compromising the reproducibility of the test, although densitometric blot analysis techniques and automated scanning and scoring attempt to address some of these limitations. Like the ELISA, Western blot can have false-positive results in healthy individuals without tick exposure, as nonspecific IgM immunoblots develop faint bands. This is because of cross-reaction between B burgdorferi antigens and antigens from other microorganisms. Around 50% of healthy adults show low-level serum IgG reactivity against the FlaB antigen, leading to false-positive results as well. In cases in which the Western blot result is indeterminate, other etiologies must be considered.

    Also Check: Lyme Literate Doctors Los Angeles

    Key Points To Remember

    • Most Lyme disease tests are designed to detect antibodies made by the body in response to infection.
    • Antibodies can take several weeks to develop, so patients may test negative if infected only recently.
    • Antibodies normally persist in the blood for months or even years after the infection is gone therefore, the test cannot be used to determine cure.
    • Infection with other diseases, including some tickborne diseases, or some viral, bacterial, or autoimmune diseases, can result in false positive test results.
    • Some tests give results for two types of antibody, IgM and IgG. Positive IgM results should be disregarded if the patient has been ill for more than 30 days.

    Is Treatment 100% Effective

    How Lyme Disease Tests Are Designed Today

    Scientists are divided on this topic. Some studies suggest that that even long-term antibiotics may not completely clear infection dogs may get sick again at some point after antibiotic treatment is stopped. Other studies suggest that complete clearance of infection is possible with antibiotic treatment. Further research is required to answer this question.

    Contributors: Ryan Llera, BSc, DVM Kristiina Ruotsalo, DVM, DVSc, Dip ACVP Margo S. Tant, BSc, DVM, DVSc

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    Why Igenex For Tick

    • CLIA certified in all 50 states
    • Long track record of success since 1991
    • Offers testing that is nearly twice as accurate as CDC recommended tests
    • Tests for all major tick-borne illnesses, including Lyme disease, TBRF, Babesia, Bartonella, and Rickettsia
    • Utilizes comprehensive testing methods such as PCR, ImmunoBlots, IgXSpot and FISH
    • Has become a nationwide leader in COVID-19 testing due to a relentless focus on customer satisfaction

    Gene Sequences And Pcr Results

    Alignment, individuals values of copies/PCR, and ROC analysis of copies/PCR. Sequences published by Amouriaux et al. and by Shan et al. were aligned in MEGA 5 . The alignment shows that there is some overlap between these sequences. The overlap starts at position 102 into the sequence published by Amouriaux et al. and continues until pos 420. Dashes indicate the end of sequence used by Amouriaux. There are only two mismatches to cp32-1 and these are single base insertions. These data indicate that the cp32 terminal phage subunit gene region was already used as a target for diagnostic Borrelia PCR prior to the publication by Shan et al. Even though the mean value of copies/PCR are significantly different in our analysis between late LB and healthy individuals, there is significant overlap between all groups. This was observed both in serum and in WB. ROC curves of mean values WB and serum. Comparison of healthy volunteers to early LD. Whole blood AUC = 0.697. Serum AUC = 0.400 . Comparison of healthy volunteers and late LD. Whole blood AUC = 0.738 . Serum AUC = 0.622 . LB, Lyme borreliosis WB, whole blood ROC, receiver operating characteristic AUC, area under the curve CI, confidence interval ns, non-significant *p< 0.05, **p< 0.01, calculated using independent-samples Mann-Whitney U-test for comparing participant groups dependent-samples Wilcoxon Signed Rank test for comparing whole-blood vs. serum within a participant group.

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    Types Of Lyme Disease Tests

    Antibody titer tests for Lyme disease measure antibodies that are specific to the bacteria that can trigger the condition. Antibodies are part of the bodys immune response. Tests look for two types of antibodies, known as immunoglobulin M and immunoglobulin G .

    IgM antibodies usually peak within a few weeks after an infection with Borrelia bacteria and start to collapse 4-6 months after infection. IgG antibodies are slower to develop, beginning to show 4-6 weeks after exposure and may peak 4-6 months after exposure. While IgM tends to be associated with an active infection, both IgM and IgG can persist in the blood for many years.

    The Centers for Disease Control and Prevention recommends a two-stage or two-tiered test for these antibodies. If the initial blood test is positive for IgM or IgG antibodies associated with the Borrelia bacteria, a second test is done, often using different laboratory methods.

    Testing for IgM and IgG can support the finding that you have been exposed to Borrelia, but this on its own does not prove whether or not you have Lyme disease.

    Follow-up testing may be necessary if testing is inconclusive. This could involve repeat blood tests or the use of different types of medical tests to rule out other health conditions. In people suspected of bacterial reinfection, imaging tests may be used to detect joint inflammation.

    Positive C6 Elisa On Serum

    Lyme disease

    RIPL will automatically proceed to do IgM and IgG immunoblot tests after a positive or indeterminate ELISA test and will provide an overall interpretation of the ELISA and immunoblot in the light of the clinical details provided on the request form.

    Please provide clinical details to allow the interpretation of serological results. These are needed for interpretation because antibody levels from a protective humoral immune response to Borrelia species may persist indefinitely in patients who have had Lyme disease in the past. After successful treatment of Lyme disease antibody concentrations may slowly fall over time.

    Borrelia species are notifiable organisms. The numbers of positive results from laboratory confirmed cases in RIPL are reported to PHE and analysed for inclusion in PHE Health Protection Reports as part of Lyme disease epidemiology and surveillance.

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    Why Choose Us For Your Lyme Pcr Test

    • Since 2002, we have a proven track record of offering excellent customer service, affordable pricing with no hidden fees, and our enduring commitment to protect your personal information.
    • No doctor’s order? Our national physician network provides the required doctor’s order for the lab. Insurance is not needed to order lab testing nor will your insurance company be billed.
    • We are partnered with two of the largest CLIA certified labs in the US, to offer you the latest lab testing technology with prompt and accurate results.
    • With over 3,600 lab locations to choose from and same day collection, we make lab testing quick and convenient. No appointment is needed. Find your local lab with our location finder.
    • We offer an extensive and detailed test menu. Ordering can be done online or over the phone. Not finding a test? Simply give us a call.

    Why It Is Done

    A Lyme disease test is done to diagnose Lyme disease in people who have symptoms of Lyme disease. Symptoms may include:

    • An expanding red rash with a pale center. This is sometimes called a “bull’s-eye” rash.
    • Extreme tiredness.
    • Headache and stiff neck.
    • Muscle and joint pain.

    Symptoms of chronic Lyme disease infection include joint pain, stiffness, and problems with the heart, brain, or nerves.

    Testing is most accurate when you have risk factors for Lyme disease or symptoms of the disease.

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    Management Of Individuals Without Symptoms Following A Tick Bite

    Diagnostic testing is not recommended for individuals who do not develop any symptoms suggestive of Lyme disease after a tick bite.

    Some commercial companies offer services to test removed ticks for the presence of the bacteria that cause Lyme disease. PHE does not provide such tick-testing services. The results of such tests should not be used to inform diagnosis or treatment. A positive result does not mean that the infected tick will have passed on the bacteria there are many factors that determine whether Lyme disease results from the bite of an infected tick. A negative result may not be technically valid and could give false assurance, as it does not exclude the possibility that another tick elsewhere on the body has been missed by the patient.

    PHE runs a tick surveillance scheme and is happy to receive ticks for species identification and to monitor tick distribution.

    Who Should Get Testing

    Ask Mara: Basic Testing for Lyme Disease

    Testing is usually indicated if a person has symptoms of Lyme disease and a known or possible exposure to ticks that can carry the Borrelia bacteria. However, because it takes time for antibodies to develop, the timing of testing is important to consider.

    Lyme disease symptoms depend on the extent of the bacterial infection. Three phases are used to describe the infection:

  • Early localized: In this initial phase, symptoms are normally found only near the tick bite. This early stage is called erythema migrans and involves a noticeable rash that typically develops within a few weeks after being bitten.
  • Early disseminated: In this phase, the bacteria move through the blood to affect other parts of the body and can cause general symptoms like fever, headaches, and pain. In some cases, the bacteria can affect the heart or nervous system.
  • Late disease: This phase occurs long after the initial tick bite and most often includes symptoms affecting the joints or nervous system.
  • It is important to understand these phases because testing is not equally valuable in each stage. With early localized disease and erythema migrans, blood testing is generally not helpful because antibodies have not had enough time to develop.

    Because of similar concerns about potential false positive results, random screening for Lyme disease in people without symptoms is not recommended even in areas that are known to have ticks that can carry the Borrelia bacteria.

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    What To Think About

    • It may be hard to tell if you have Lyme disease. False-positive and false-negative Lyme disease test results are common. Many people do not make antibodies to Lyme disease bacteria for up to 8 weeks after being infected.
    • Doctors often do not rely on test results alone when recommending treatment for a person who may have Lyme disease. Treatment is often based on a person’s symptoms, the time of year, having a tick bite, and other risk factors for Lyme disease.

    Question 4 What Tests To Assist In The Diagnosis Of Lyme Disease Are Available At Quest

    Serologic testing is the principal means of laboratory diagnosis of Lyme disease. Quest offers testing in accordance with the Centers for Disease Control and Prevention guidelines for early/acute Lyme testing.3 When laboratory diagnosis is indicated, current recommendations include using a 2-tier testing approach that begins with a sensitive enzyme immunoassay , followed by a confirmatory immunoassay for specimens yielding positive or equivocal results.3 In the standard 2-tier test algorithm, a Western blot or immunoblot assay is used for confirmation. However, on July 29, 2019, the US Food and Drug Administration cleared several Lyme disease serologic assays with new indications for use, which allowed an EIA to be used as the confirmatory test in a modified 2-tier testing algorithm.3 The MTTT algorithm is now considered an acceptable approach for the serologic diagnosis of Lyme disease and may be able to assist in the identification of early Lyme disease within the first 30 days of infection.3

    Quest offers test options that use both the STTT and the MTTT algorithm:

    • STTT: Lyme Disease Ab with Reflex to Blot
    • MTTT: Lyme Disease Antibody with Reflex to Immunoassay

    Click here for a complete list of additional tests for tick-borne infectious diseases available from Quest.

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