Subculture Studies To Evaluate The Activity Of Natural Product Extracts Against Stationary Phase B Burgdorferi
To confirm the activity of the natural product extracts in eradicating the stationary phase B. burgdorferi cells, we performed subculture studies as previously described . We further tested the top active natural product extracts to ascertain if they could eradicate stationary phase B. burgdorferi cells at 1 or 0.5% by subculture after the treatment . Treatment with 1% Cryptolepis sanguinolenta extract caused no regrowth in the subculture study . However, the other natural product extracts including Polygonum cuspidatum, Artemisia annua, Juglans nigra, and Uncaria tomentosa could not eradicate B. burgdorferi stationary phase cells as many spirochetes were still visible after 21-day subculture . At 0.5%, all the natural product extracts treated samples grew back after 21-day subculture , however, only one of the three Cryptolepis sanguinolenta extract treated samples grew back. This indicates that 0.5% Cryptolepis sanguinolenta extract still has strong activity and could almost eradicate the stationary phase B. burgdorferi cells. By contrast, the clinically used antibiotics doxycycline and cefuroxime at clinically relevant concentration could not sterilize the B. burgdorferi stationary phase culture, since spirochetes were visible after 21-day subculture .
Natural Remedies For The Chronic Inflammation Of Lyme Disease
Everyone knows that inflammation isnt good, especially when it becomes chronic. But to do something about it, you need to understand whats driving the inflammation in the first place.
Though you cant actually see chronic inflammation, you can certainly feel it. It manifests as joint discomfort, stiffness, general achiness, fatigue, low stamina, brain fog, slow mental activity, depressed mood, and all the other symptoms associated with chronic Lyme disease. Simply put, you feel inflamed.
The root of inflammation is excessive turnover of cells. We all lose cells, and up to a certain point, its perfectly normal. Cells in the body are constantly wearing out, getting injured, or being invaded by microbes. In fact, we typically lose 50-70 billion cells every day. While much of cellular turnover is accounted for by cells that are shed from the body, such as skin and intestinal cells, tissues inside the body are losing cells, too.
When cells die, they break apart and create debris. If enough debris collects in tissues, it obstructs the flow of water, nutrients, and oxygen that cells need to stay healthy. At the same time, metabolic waste produced by cells is trapped around the cell, which, of course, isnt a good thing. If enough debris collects, cells start to choke in their own waste.
How Herbal Supplements Can Help
The best way to gain the level of phytochemical protection you need to help your immune system win the internal battle against invasive microbes is to take herbal supplements specifically, standardized botanical extracts.
Standardized botanical extracts take herbal supplements to the next level. Theyre made with wild plants or plants that have been cultivated to enhance their phytochemical content and are grown in an environment that doesnt introduce unwanted chemical toxins. The extraction process also yields the greatest concentration and the widest spectrum of phytochemicals in the plant. And the final product is standardized to a concentration of one or several key phytochemicals in the plant, so you know what youre getting.
Below are a few of my favorite herbs for mitigating the systemic chronic inflammation associated with chronic Lyme disease. Though all of the herbs have value in suppressing microbes and reducing inflammation, three of the herbs mentioned below Japanese knotweed, cats claw, and Chinese skullcap were found in a 2020 study from Johns Hopkins University to have greater activity against Borrelia burgdorferi than the antibiotics, doxycycline and azithromycin.
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When Inflammation Becomes Chronic
Imagine if the acute inflammatory process was going on in tissues throughout your body and it never resolved. Thats exactly what happens with the chronic inflammation associated with chronic Lyme disease.
The driving force is bacteria invading cells. Its not an all-out invasion like you would find with an acute infection, such as pneumonia, however. Its an insidious migration of bacteria into tissues throughout the body in the brain, heart, joints, muscles, kidneys, intestines, everywhere.
When Lyme bacteria enter the bloodstream from a tick bite, WBCs gobble up the bacteria in an attempt to get rid of them. The bacteria, however, have sophisticated ways of staying alive inside the WBCs. Instead of eradicating the bacteria, the WBCs unintentionally become transport vehicles to distribute bacteria to tissues throughout the body.
On arrival, the bacteria surface and infect other cells. Its not as much a deluge of invasion as it is a random sprinkling. Tissues throughout the body become peppered with cells that have been infected with bacteria.
The Lyme bacteria, Borrelia burgdorferi, are intracellular, which means they infect and live inside cells. The cells provide nutrients to make new bacteria as well as protection from the immune system. Once the bacteria have used up the resources a cell has to offer, they surface and infect other cells.
Western Blot Analysis Of P
To verify gene induction, one of the PXR-driven genes, P-gp, was also analyzed at the protein level. Moreover, since the regulation of CYP2J2 is largely unknown, its up-regulation by Samento was also investigated at the protein level. Protein expression was quantified by SDS-PAGE and western blotting according to standard protocols as published previously . Protein detection was carried out with a murine monoclonal antibody against human P-gp , or human CYP2J2 , or -actin . After extensive washing, blots were incubated with horseradish peroxidase-linked secondary antibodies and bands were visualized by chemiluminescence using the Pierce ECL Western Blotting Substrate and semi-quantified by FluorChem Q SA AlphaView Version 3.2.2, Cell Biosciences . Expression was normalized to the loading control -actin and the untreated medium control. Each blot was conducted in quintuplicate.
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Herbal & Prescription Lyme Disease Antibiotics
In this article, you will learn how to build a Lyme disease herbal or prescription antibiotic treatment. Later, in this guide I provide nine sample herbal and prescription antibiotic treatment plans, including the dosing regimens I find effective.
The focus of this article is on Lyme germ treatment. For information about treating Lyme disease coinfections see
Before I describe these plans, you should be aware that chronic Lyme disease recovery requires more than antibiotics. I cannot emphasize this point strongly enough. Treating Borrelia, the Lyme germ, with antibiotics is complicated because limited research shows which treatment regimens work best. As a result, many Lyme-Literate Medical Doctors , like me, prescribe antibiotics based on theory, in addition to our collective observation, which is that combinations of antimicrobials work better than single agents alone. It is critical that a Lyme disease treatment regimen addresses the first ten steps outlined in my Lyme disease treatment guidelines, The Ross Lyme Support Protocol. These steps are designed to revive health and to boost the immune system. Even a year or more into treatment, when a person is feeling better, these steps are essential to speed recovery by supporting the immune system.
Inhibition Of Cyp1a2 Cyp2b6 Cyp2c19 And Cyp3a4
Inhibition of CYPs was assessed with the P450-Glo Screening Systems according to the manufacturers instructions. The kits contain a luminogenic substrate , which is converted by the respective enzyme into luciferin. When incubated with the luciferin detection reagent, the luciferase generates light. Banderol and Samento were tested for their capacity to inhibit the production of the luminescent signal. Positive controls used were ketoconazole for CYP3A4, omeprazole for CYP2C19, miconazole for CYP2B6, and -naphthoflavone for CYP1A2. Results are not depicted, because the potency of a small molecule cannot be compared to that of an extract .
Eight concentrations in triplicates ranging from 0.0033 to 1% were tested and each experiment was conducted 35 times.
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Natural Remedies To Tame The Fires
Eating a healthy diet, living in a clean environment, learning to live around stress, and staying active are all very important for keeping cells in your body healthy. However, the chemical substances in plants defined as herbs can take that protection to the next level. Taking herbs can give you the advantage that you need to overcome chronic inflammation and return to a normal life.
Plants must protect their cells from a wide range of stress factors: damaging free radicals, physical stress from harsh weather, toxic substances, harmful radiation, insects, invasive fungi, parasites, and, last but not least, every variety of microbe.
Plants do this with their own natural chemistry. In fact, plants are the most sophisticated chemists on the planet. The chemical substances that plants use to solve problems and protect cells are called phytochemicals.
Mushrooms, which are technically fungi, also have to deal with similar stress factors as plant. They also produce a wide range of protective substances that mirror the protective properties of phytochemicals found in plants.
When we consume plant phytochemicals, the benefits are transferred to us. The three primary things that phytochemicals do for us:
Can Lyme Disease Be Prevented
There’s no sure way to avoid getting Lyme disease. But you can minimize your risk. Be aware of ticks when you’re in high-risk areas. If you work outdoors or spend time gardening, fishing, hunting, or camping, take precautions:
- Wear closed shoes or boots, long-sleeved shirts, and long pants. Tuck your pant legs into your shoes or boots to prevent ticks from crawling up your legs.
- Use an insect repellent containing 10% to 30% DEET .
- Wear light-colored clothing to help you see ticks more easily.
- Keep long hair pulled back or wear a hat for protection.
- Don’t sit on the ground outside.
- Check yourself for ticks regularly both indoors and outdoors. Wash your clothes and hair after leaving tick-infested areas.
If you use an insect repellent containing DEET, follow the directions on the product’s label and don’t overapply it. Place DEET on shirt collars and sleeves and pant cuffs, and only use it directly on exposed areas of skin. Be sure to wash it off when you go back indoors.
No vaccine for Lyme disease is currently on the market in the United States.
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In Vitro: Effectiveness Of Samento And Banderol Herbal Extracts On The Different Morphological Forms Of Borrelia Burgdorferi
|Figure 1A: Biofilm
|Figure 1B: Biofilm after exposure to Doxycycline — causes biofilm to increase in size.
|Figure 1C: Biofilm after exposure to Samento and Banderol — there was a complete elimination of biofilm.
Samento & Banderol Herbal ExtractsCorresponding Author Eva Sapi, PhD University of New Haven Department of Biology and Environmental Sciences300 Boston Post Road West Haven, Connecticut 06516 firstname.lastname@example.orgReferences
Mic Values Of The Active Natural Product Extracts
Because the activity of antibiotics against non-growing B. burgdorferi is not always correlated with their activity against growing bacteria , we therefore determined the MICs of these natural product extracts against the replicating B. burgdorferi as described previously . The MIC values of some natural product extracts such as Artemisia annua, Juglans nigra, Uncaria tomentosa were quite high for growing B. burgdorferi, despite their strong activity against the non-growing stationary phase B. burgdorferi cells . On the other hand, the top two active natural product extracts Cryptolepis sanguinolenta and Polygonum cuspidatum showed strong activity against the growing B. burgdorferi with a low MIC and also non-growing stationary phase B. burgdorferi .
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My Recommended Readings For Treating Lyme Disease
If you want to read more information regarding natural ways to treat Lyme disease, then I highly recommend Dr. Buhner’s book “Healing Lyme”! I have read it, and I refer to it often. He is an expert in the field and explains everything well in the book. I am also including a link for you to obtain another book if you prefer to read on your Kindle . I have not read this book, but it has been highly recommended in my research. The third book that I am recommending is also very good. It has information regarding combined tradition and herbal therapies.
Samento And Banderol: Uses And Research
Proponents of banderol and samento recommend them as alternatives to antibiotics, which are the standard treatments for Lyme disease. It’s said that these herbal remedies may help patients steer clear of the side effects sometimes associated with antibiotic use, such as gastrointestinal distress.
Supporters also say that banderol and samento aid in the treatment of Lyme disease by reducing chronic inflammation.
In addition, banderol and samento are sometimes used to control post-treatment Lyme disease syndrome . In people with PTLDS, symptoms linger long after they’ve completed a course of antibiotics. These symptoms include fatigue, muscle and joint pain, sleep disruption, and mood changes.
At this point, however, theres very little evidence to back up claims that banderol or samento can help treat Lyme disease. There is some research out there showing that a combination of samento and banderol may help knock out Borrelia burgdorferi, the bacterium responsible for Lyme disease. One study often pointed to, however, lacks rigorous peer review. Scientists have yet to confirm these findings or those of related research in clinical trials involving Lyme disease patients.
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Chronic Disease Can Be Managed
Like many in the Hudson Valley, Lyme disease has turned the Callahans’ lives upside down. Both of their children are afflicted with the illness, which has proven to be a powerful foe for their youngsters to fight.
“Lyme disease has impacted our lives on and off for five years, and daily for over a year,” Laura Callahan said, who lives in Milton, Ulster County. “My son Ryan was unable to attend school for most of last year. He spent the majority of his day in bed with excruciating headaches and severe fatigue.”
Unfortunately, the challenges the Callahans face are not uncommon. More than 300,000 Americans are diagnosed with Lyme disease annually, according to the Centers for Disease Control and Prevention. Of those infected, 96 percent are in 13 states, including New York, with the highest rates reported in Columbia, Dutchess, Greene, Putnam, Orange and Ulster counties.
Dr. Richard I. Horowitz, author of the New York Times best-seller, “Why Can’t I Get Better? Solving the Mystery of Lyme and Chronic Disease,” and medical director of the Hudson Valley Healing Arts Center in Hyde Park contends that the method used to calculate the 300,000 figure is flawed. It doesn’t take into account the fact that Lyme is a multi-systemic malady.
Experts say 25 percent of Lyme cases in the Hudson Valley are children.
Under-reporting and misdiagnosis in children is due in part to the fact they often aren’t able to describe their symptoms accurately.
Symptoms in children with Lyme disease
Healthy Vs Weak Cells
How all this plays out is a function of the health of cells as much as the aggressiveness of the bacteria: Cells that are weak from being chronically stressed are more vulnerable to invasion by bacteria.
A healthy person with healthy cells who is bitten by a tick may not have much in the way of acute symptoms and may never develop chronic symptoms. It doesnt mean, however, that the bacteria have been eradicated from the body. They can stay dormant in tissues for a lifetime.
All it takes is a shift in the health of cells for dormant bacteria to surface and invade more cells. The immune system scrambles to keep a lid on things as bacteria erupt and infect other cells. Dead cells break apart and create debris. Macrophages secrete acid and free radicals to break down debris. Reinforcements are called in. More acid. More free radicals. More collateral damage. More debris. Flow of nutrients and oxygen is obstructed and cells are weakened even further.
The invasion intensifies as more cells are infected. Along with inflicting injury to cells in tissues, bacteria infect and kill WBCs. In addition, bacteria throw the immune system off balance by disrupting the cytokines the immune system uses to coordinate the resistance. As the process spirals out of control, the immune system loses the capacity to keep microbes in check.
Before long, tissues throughout the body start to look like a war zone. Cells suffer. Symptoms result. It becomes a never-ending cycle of misery.
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Macrolide Plus Quinine Derivative Plus Azole Plus Methylene Blue
- azithromycin 500 mg 1 pill 1 time a day
- hydroxychloroquine 200 mg 1 pill 2 times a day
- metronidazole 500 mg 1 pill 2 or 3 times a day
- methylene blue 50 mg 2 times a day
- Since azithromycin is not as effective as other macrolides like clarithromycin, hydroxychloroquine is used to increase the effectiveness.
- Tinidazole could be substituted for the metronidazole because it may remove biofilms more effectively.
- Methylene Blue can treat persister Lyme.